8^th World Congress on
Immunology

Biography:

Steven Osterhout DC, CCN is the founder and director of Vitality Healthcare, an integrative medical and natural healing center catering to identifying the underlying causes of poor health with remarkable positive patient outcomes.  He attained is Pre-Med from Western Michigan University, going on to earn his Doctorate from Palmer, as well as a postdoctoral Board Certification in Clinical Nutrition.  He is a member of the Academy of Anti-Aging Medicine, Academy of Environmental Medicine, and the Institute for Functional Medicine.  He is the published author of The Diabetic Doctor and has over twenty years of experience traveling the world investigating the most favorable approaches to accelerate the healing process.

Abstract:

Multiple inflammatory mechanisms are activated by today’s lifestyle choices and environmental exposures. Common consequences include reduced immune function as well as loss of immune tolerance.  Environmental exposures to common chemicals like bisphenol A, parabens, phthalates and other toxicants drive endocrine disruption.  These estrogen mimicking compounds support a physiology of estrogen dominance and contribute to molecular mimicry.   Constant daily exposure not only stifles immune function it also opens the door to inflammatory dysregulation and autoimmune behavior.  Increased inflammatory responses combined with perpetual feedback inflammatory cycles creates short term acute symptoms while manifesting under-recognized associated chronic degenerative disease expression.  These inflammatory daily exposures provide an underlying contribution to gastrointestinal dysfunction called hyper-permeable bowel and neurological permeability.  While governmental chemical regulation is sluggish in response to these common toxicants, modifying personal exposure and limiting bioaccumulation will decrease potential of autoimmune activation.  Observation, modification, and appropriate choices of lifestyle exposures may present a powerful tool in quieting inflammatory degenerative processes.

Biography:

Abstract:

Methods of research In vivo research of therapeutical effect in 
hamsters with experimental myeloid tumor of Graffi (Toshkova, 

Drossinakis, 2018).The research is co-written with Prof. RenetaToshkova, Ass. Prof. Elissaveta Zvetkova и Ass. Prof. Georgi Gluhchev.

In over 80 institutes and centers in Europe has been proved the strong bio-influence of Drossinakis via the application of the methods biophotons (Popp), thermovision (Schlebusch), spectrum analysis of water (Antonov, Ignatov), color coronal spectral analysis (Ignatov), gas discharge visualization (Korotkov), synchronizing of brain electromagnetic waves (Li Gendinovich) etc. Drossinakis has achieved the following results according the norm – biophoton emission (Popp) (~900 biophotons 1 cm2/ 1s), (norm ~85 photones, 1 cm2/1s); increasing of the temperature after bioinfluence (Schlebusch) (1.6-1.8 °Ð¡) (norm 0.1°Ð¡); average energy of hydrogen bonds among water molecules according control sample (Antonov, Ignatov) (±8.2 meV) (±1.1 meV) etc.

In the current study was followed the effect of influence with Infrared thermal field (ITF) and electromagnetic fields (e.m. fields) of Christos Drossinakis over experimental myeloid tumor of Graffi, implanted in hamsters. The study was conducted by a team under the authority of Reneta Toshkova.

The working hypothesis (concept) of Drossinakis for treatment of tumors includes several facts – redistribution of the energy in the ill and healthy zones of the body, change of ion balance at molecular level and improved interchange in the cell membranes.

The author’s team of Reneta Toshkova, Ignat Ignatov, Elissaveta Zvetkova and Georgi Gluhchev together with Christos Drossinakis, has conducted experimental research in model systems.

Methods of research

1. In vivo research of therapeutical effect in hamsters with experimental myeloid tumor of Graffi (Toshkova, Drossinakis, 2018).

Two types of trials were conducted for bio-influence of experimental myeloid tumor in hamsters with ITF and e.m. fields.

In the first set of trials the bio-influence was applied over a tumor with size around 10 mm in diameter. In the second set of trials the influence was carried out together with the transplantation of the tumour. The results were compared with two control groups – hamsters with untreated transplanted tumors and healthy animals.

As indicators for assessment of the therapeutical effect under the influence of ITF and e.m. fields were reported parameters such as – tumor appearance, tumor growth, lethality, survival rate and average survival rate.

2.Research of 1% solution of blood serum of hamsters with tumors using the spectral methods NES and DNES. (Ignatov, Drossinakis, 2018).

3. Analysis of the biologocal effects in peripheral blood of hamsters with tumors after the influence (Zvetkova, Drossinakis, 2018).

4. Examination of physiological saline solution (Gluhchev, Drossinakis, 2018).

Results

1. In both experiments for bio-influence over hamsters-tumor carriers were observed the following results.

- The average survival rate of hamsters with bioinfluence is 43.6±5.8 days, for control group-without bioinfluence – 31.75±6.8 days, i.e. the survival rate in the first group is prolonged with around 12 days.

- The average survival time (AST) of hamsters with ITF and e.m. waves has been established compared to the control group untreated animals. The hamsters from group with bioinfluence survive 49.0±6.63 days, and from control group - 33.25±6.29 days, which is around 16 days in favor of hamsters with ITF and e.m. waves.

As a conclusion, it is observed slowed tumor growth resulting in – prolonged survivability in the second set experimental set.

2. In the spectrum analysis with method NES conducted by Ignatov were achieved substantial results from the influence with ITF and e.m. waves compared to the control group of hamsters with tumor and healthy hamsters. In the spectrum analysis was measured the energy of the hydrogen bonds H...O among water molecules. Under the influence of ITF and e.m. fields, the result was E=-0.1285 eV for blood serum of hamsters with tumor. In the control group of hamsters with tumor it was E=-0.1214 eV, and in healthy hamsters it was E=-0.1250 eV. The spectrum DNES was achieved using the formula

∆f(E) = f (sample ) – f (control sample).

The result against control group of cancer hamsters was ∆E=-0.0071±0.0011 eV, and against the control group of healthy hamsters was ∆E=-0.0035±0.0011 eV. Substantial results were achieved in the analysis of local extremums in the water spectrum. For E = -0.1212 eV (λ = 10.23 μm; 978 cm-1) the result is indicative for anti-inflammatory effects. For E = -0.1387 eV (λ = 8.95 μm; 1117 cm-1) the result is connected to supression of the development of tumor cells in size and number.

3. Conclusions deducted by Zvetkova. When studying the swabs of peripheral blood of the experimental hamsters was established a tendency for positive influence over the erithropoesis and the anemic syndrome accompanying tumor development.

4. During examination of physiological saline of NaCl from Gluhchev was observed five-fold increase of hydrogen ions and change of conductivity. It is a proof for recovery of the ion balance. In healthy cells the potential for transmission of hydrogen ions H+ through the membrane is (-140 mV), and in cancer cells it is (-70 mV)(Alberts et al.). The tendency is that under the influence of ITF and e.m. waves the potential can be recovered.

Conclusion: The achieved results of hamsters from experimental bio-influence of Christos Drossinakis reveal their biological efficiency and can be subject of future studies. Extending the life of the hamsters is an indicator of improving immune status. The results obtained with recent data in the medical scientific literature on the positive effect of the near infrared irradiation on the structure and function of erythrocyte membrane in normal and pathological conditions. The mitochondrial polarity in cancer cells was found to be lower than that of normal cells. Drossinakis is increasing the mitochondrial polarity.

Biography:

Abstract:

This study was conducted to investigate the effects of different levels of a water-soluble polysaccharide extract from brown algae, Padina australis (WPP) on growth, antioxidant enzymes activities and nonspecific immune responses of shrimp, Litopenaeus vannamei . Three replicate groups of shrimp (1 ± 0.1g) were fed four isonitrogenous and isolipidic diets containing four levels, 0 or control, 0.5, 1.0, and 1.5 g kg^-1 of WPP for 8 weeks. Results show that the diet containing 1.0 g kg^-1 of WPP had a significant positive effect on growth performance of shrimp. In addition, the activities of antioxidant enzymes including superoxide dismutase, glutathione peroxidase, catalase and malondialdehyde improved by feeding 0.5 and 1 g kg^-1 of WPP. Also, the lysozyme and phenoloxidase activities in those of shrimp fed 0.5 and 1 g kg^-1 of WPP were significantly higher than those fed control and 1.5 g kg^-1 WPP. These results suggested that the use of 1.0 g kg^-1 of water-soluble polysaccharides of P. australis can be beneficial for growth, antioxidant and nonspecific immune of L. vannamei.

Keywords: Water-soluble polysaccharides, Padina australis, Litopenaeus vannamei, Antioxidant enzyme and Immune response.

Biography:

Abstract:

The objective of the study was to examine the effects of dietary supplementation of Chlorella vulgaris powder (CP) on growth, antioxidant enzyme activity, immune responses of juvenile Mugil cephalus. Fish (15 ± 0.1g) were fed diets enriched with 0 (control), 5, 10 and 15 g CP per kg feed for 8 weeks. After the feeding trial, fish were challenged against pathogenic bacteria (Photobacterium damselae) infection to evaluate disease resistance of infected fish. Fish growth performance was significantly improved with increasing CP levels at CP10 and CP15. Antioxidant-stimulated activity was observed with dietary CP where total antioxidant capacities (TAC), superoxide dismutase (SOD) and glutathione (GSH) increased, while malondialdehyde (MDA) decreased significantly in fish fed CP. Furthermore, serum lysozyme activity of fish fed three levels of CP was significantly higher than control group. In addition, CP supplementation at 10 and 15 g dose induced kidney phagocytic and respiratory burst activity which was maximized at 15 g CP. Meanwhile, feeding 10 and 15 g of CP diets decreased mortality in M. cephalus after challenge with P. damselae.

Keywords: Chlorella vulgaris. Mugil cephalus . Antioxidant enzyme . Immune response.

Biography:

Abstract:

This study was conducted to investigate the effects of different levels of water-soluble polysaccharide extract from brown algae, Padina australis (WPP) on growth, antioxidant enzymes activities and nonspecific immune responses of shrimp, Litopenaeus vannamei .Three replicate groups of shrimp (1 ± 0.1g) were fed four isonitrogenous and isolipidic diets containing four levels, 0 or control, 0.5, 1.0, and 1.5 g kg^-1 of WPP for 8 weeks. Results show that the diet containing 1.0 g kg^-1 of WPP had significant positive effect on growth performance of shrimp. In addition, the activities of antioxidant enzymes including superoxide dismutase, glutathione peroxidase, catalase and malondialdehyde improved by feeding 0.5 and 1 g kg^-1 of WPP. Also, the lysozyme and phenoloxidase activities in those of shrimp fed 0.5 and 1 g kg^-1 of WPP were significantly higher than those fed control and 1.5 g kg^-1 WPP. These results suggested that the use of 1.0 g kg^-1 of water-soluble polysaccharides of P. australis can be beneficial for growth, antioxidant and nonspecific immune of L. vannamei.

Biography:

Dr Teda is a general practitioner who has the passion in immunology and infection. She freshly graduate from medical school after completing this research. 

Abstract:

Statement of the Problem: Tuberculosis (TB) is one of the top 10 causes of death worldwide. The severity of pulmonary TB symptoms is affected by the immune status which is strongly influenced by nutrition. Immunonutrient can be defined as the effect of the provision of specific nutrients on immune function. Vitamin D is known to enhance immune response to mycobacteria. In addition, protein products cytokine which help body defense to mycobacteria. The aim of this study was to determine the effect vitamin D and protein intake as the immunonutrient on severity of pulmonary tuberculosis clinical symptoms. Methodology & Theoretical Orientation: The method of this study is cross sectional study of 52 pulmonary tuberculosis patients. They were interviewed with questionnaire of FFQ. The amount of vitamin D and protein intake is calculated by using the nutrisurvey. Clinical symptoms of TB patients are measured by using Bandim TBScore. Statistical analysis of the two variables is allegedly linked using SPSS. Findings: The average intake of vitamin D is 5.86 mcg/day, SD 5.45 . The average intake of protein is 78,477 gram/day, SD 29,30. The respondents who have degree TBScore I is 92.3% (48 persons), TBScore II is 7.69% (4 persons),  TBScore III is 0% (0 persons). The average of TBScore is 2,04 ± 1,89. Conclusion & Significance: Based on the statistical analysis, vitamin D and protein intake are not significantly affect the severity of pulmonary TB clinical symptoms. However, the average intake of TBScore II group is much lower than the intake of TBScore I group. 

Biography:

Abstract:

This work was instigated by a certain comparative study drawn on immunogenic biological therapy and its clinical relevance on Psoriatic Arthritis delivered in a conference in 2016. The work which projected a comparative biological understudy of anti-inflammatory biologic activities as a catalysis on significant determinant in addressing diseases life activities in Psoriatic arthritis (PsA) especially, emphatic in the cases of joint infection, held that the residues of protein presences in biologics pre-disposes the capacities to re-create anti-drug antibodies (ADAbs) which may stimulate clinical investigation result. However, a critique look on the disparity of levels and frequencies of occurrences of ADAb formations, potentiate effects on safety and efficacy of texts, journals and articles sighted, theirs and others demonstrated a different level of result and outcome throwing up reason for further questions.

Biography:

Practice as a pharmacist for 7 Years currently the Senior Pham. At the Edo Pharmaceutical Limited Graduated from Ambros Alli University Ekpoma, Edo state of Nigeria am recently on a research trying to find solution to safe child vaccination procedures in our primary Health Care facilities in the rural area 

Abstract:

This Idea was born out of numerous deaths from in proper immunization and vaccination practices against certain diseases because of immunodeficiency. We look deeply into our local primary health care system and we found out there are many ways we could develop a new system of vaccination that will deal with unprofessionalism and eradicate unhealthy processes. This work which will elaborates on information’s concerning activities that has increased infectious diseases from wrong vaccination practices and activities, Vaccination is a critical practice to enhance healthy leaving and increase on war against diseases and infections. Our findings which is on a large print will bring about ways we should develop on vaccination process and integrated certain resent technologies that will simplify immunisation and vaccination process, that will portrait certain results bringing about ways to find solutions and demonstrating a way to achieve different result from our recent practices.

Biography:

Dr. Olga Kubassova

CEO of IAG, Image Analysis Group

A mathematician with over 10 years expertise in actively managing innovation in life science companies, Olga Kubassova, PhD, is a healthcare innovator and biotech investor with passion for improving people’s health. She has co-authored over 60 publications, books and book chapters, has become a scientific adviser to the UK government and EU funding bodies. She is a founder and CEO of IAG, Image Analysis Group, the imaging clinical research organization working with top bio-pharma companies to maximize their chances of brining novel therapeutic agents to the market. She is a four-time winner of national and international "Entrepreneur of the Year" awards. Olga’s ambition is to bring truly disruptive technologies, artificial intelligence and best of machine learning to clinical practice and research, while expanding IAG's footprint and partnerships.

Abstract:

Cancer treatment is reaching a breakthrough due to the advances in Immunotherapy. The fundamental technology behind immunotherapy is to harness the body’s own immune system to attack cancer cells. The original purpose of immune cells is to identify and destroy dangerous cells. However, cancer cells have developed the ability to hide from the immune system and therefore uncontrolled cell proliferation occurs which leads to tumour growth. In principle, immunotherapy makes cancer cells visible to the immune system. Therapies address both: they target the body’s immune cells to identify cancer cells and enable them to destroy the dangerous cells. Therefore, immunotherapies are also called targeted therapies.For the first time in cancer treatment it is possible to address tumour heterogeneity in a more specific way. Immunotherapy enables us to study and manipulate the tumour microenvironment. However, to evaluate, proof and leverage the potential of immunotherapies, it is essential to find methods which can show the changes in the tumour microenvironment non-invasively.This is where advanced medical imaging followed by computer-based analysis becomes paramount. Advanced magnetic resonance imaging (MRI) in combination with algorithms to calculate quantitative imaging biomarkers offer the unique opportunity to provide sensitive and specific tumour measurements. Especially in clinical research studies which aim not only to test the safety of a drug but also the efficacy in a certain patient population, the sensitive assessment of the therapy induced tumour microenvironmental changes are crucial for bringing the drug to the market.MRI of breast cancer patient with large tumor visible in the post-contrast image; At the post treatment follow-up MRI exam, the size of the tumor did not reduce significantly. At this stage, the images were processed with  AI-driven algorithm (DYNAMIKA platform) to quantify tumor vascularity and permeability, and show tumor micro-environment driven changes, as response to the therapy.