Department of Marine Sciences, Chabahar Maritime University, Fisheries group, Chabahar, Iran
Title: Effect of dietary supplementation of Chlorella vulgaris on growth, antioxidant defense and immune status of grey mullet, Mugil cephalus
The objective of the study was to examine the effects of dietary supplementation of Chlorella vulgaris powder (CP) on growth, antioxidant enzyme activity, immune responses of juvenile Mugil cephalus. Fish (15 ± 0.1g) were fed diets enriched with 0 (control), 5, 10 and 15 g CP per kg feed for 8 weeks. After the feeding trial, fish were challenged against pathogenic bacteria (Photobacterium damselae) infection to evaluate disease resistance of infected fish. Fish growth performance was significantly improved with increasing CP levels at CP10 and CP15. Antioxidant-stimulated activity was observed with dietary CP where total antioxidant capacities (TAC), superoxide dismutase (SOD) and glutathione (GSH) increased, while malondialdehyde (MDA) decreased significantly in fish fed CP. Furthermore, serum lysozyme activity of fish fed three levels of CP was significantly higher than control group. In addition, CP supplementation at 10 and 15 g dose induced kidney phagocytic and respiratory burst activity which was maximized at 15 g CP. Meanwhile, feeding 10 and 15 g of CP diets decreased mortality in M. cephalus after challenge with P. damselae.
Keywords: Chlorella vulgaris. Mugil cephalus . Antioxidant enzyme . Immune response.